Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Then the suspension was centrifugated at rpm during ten minutes. Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis.

Fusion de protoplastos pdf

The latter has a natural resistance to late blight, Phytophthora infestans Mont. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding.

Potato, Solanum tuberosum L.

Improvements in regeneration from protoplasts of potato and studies on chromosome stability. A slot-blot hybridization method for screening somatic hybrids. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Ruiz de Galarreta y E. Somatoclonal variation in potato: Interspecific protoplastso hybridization between Solanum tuberosum L. The osmolality of this medium was fixed at mOsm.


The hybridity of the xe regenerants was confirmed by RAPD markers. New York, NY Production fusiin analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. Somatic potato hybrids have been produced by fusion of diploid wild species with tetraploid S. The morphology of fusion-derived regenerants was intermediate between the donor parents. Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S.

Cultivo y fusion de protoplastos

Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml. Genetic Resourses Conservation and Characterization Division.

Introduction Potato, Solanum tuberosum L. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur. Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type.

Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Isolation of plant DNA from fresh tissue.

Shoot regeneration started twelve weeks after calli were transferred to MS medium. Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents. Somatic hybridization in the genus Solanum: A rapid calli proliferation was observed after colonies developed. Somatic hybrids obtained by protoplast fusion between Solanum tusin L.


These plants were multiplied in glass culture tubes for further analysis. In this paper we describe the fusion of leaf protoplasts of S. It has been demonstrated that protoplast-derived plants show a dw degree of chromosomal number variation than those derived from tissue or organ culture. Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Interspecific somatic hybrids between wild potato Solanum acaule Bitt. The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at rpm during five minutes.

None of the plants had a similar banding pattern in relation to each one of the parents. Somatic hybrids were confirmed morphologically under greenhouse conditions, when protoplasos characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

The same primer amplified four specific bands of, and bp in S.